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Malachite green colloidal gold method rapid detection kit

This product is used to quickly detect malachite green (MG) and crystal violet (GV) residues in aquatic tissue samples. The whole testing process is about 40 minutes, which is suitable for all kinds of enterprises and testing institutions. The detection limit of MG and GV of this product is 2ppb.

Kit composition

Specifications: 10 times / box

10 malachite green rapid detection cards (included: detection card,   micro-hole)

MG Extract Sol.1: 1 bottle

 MG Extract 2: 1 bottle

Oxidizer 1 bottle

MG special complex   solution 1 bottle

Manual Instruction

Applicable equipment and Buffer solvent

Homogenizer (scissors), balances, centrifuges, nitrogen (air) air dryers, micropipettes and matching tips.

[Sample processing]

Tissue samples (shrimp should be thoroughly cleaned after removing the head and shell, and the fish should be washed after removing the scales). It should be stored in the dark (within 24 hours). The sample is processed as follows:

1. Take a chopped amount of de-fat tissue sample and homogenize it with a homogenizer;

2. Weigh 2 g of homogenous tissue in a 15 ml centrifuge tube;

3. Add 3 ml of MG extract Sol 1 to the above centrifuge tube, add 4 ml of MG extract 2 solution, cover with vigorous shaking for 3 min, and centrifuge at room temperature for 4000 min at 4000 rpm;

4. Pipette 1.5 ml of the supernatant into a 5 ml centrifuge tube, add 50 μl of oxidant, mix and dry at 65 ° C in a nitrogen (air) air bath.

5. Add 300 μl of MG special complex solution to the blown centrifuge tube, and shake vigorously for 30 s on the vortex mixer to obtain the sample solution.

Detection steps

Read the instruction manual thoroughly before testing, and return the test card and the sample solution to be tested to room temperature (20 ° C ~ 30 ° C) before use.

Remove the test card from the original bag and place it horizontally on the front of the observer, as shown below (use it immediately after opening);

Pipette 100 μl of the sample to be added to the gold standard microwell, gently blow 30 S, wait for the reaction for 5 minutes, then all the solution in the suction well is added to the sample well;