Home> Products > Dairy Diagnostic

Beta-lactam+Tetracycline+Chloramphenicol+ Streptomycin (96 tests)

The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Beta-lactam+ Tetracycline+ Chlorampenicol+ Streptomycin (Dairy Quadrobiotic BTCStrep) in samples, it will combine with marked antibodies, preventing the marked antibodies from combining the (Dairy Quadrobiotic BTCStrep) antigens of NC nitrocellulose membrane.



Product Name

MTUSBIO (4 in 1) Beta-lactam+Tetracycline+Chloramphenicol+ Streptomycin (96 tests)


Specification

96T/kit


Storage Conditions

Store at 2-8℃   in dry environment. / Expiry date: 12 months


Intended Application

Beta-lactam+Tetracycline+Chlorampenicol+Streptomycin (Dairy QuadroBiotic BTCStrep: Penicillin G, Cefquinome, Ampicillin, Cefacetrile, Amoxicillin, Cefalonium, Oxacillin, Cefazolin, Cloxacillin, Cefoperazone, Dicloxacillin, Cephapirin, Nafcillin, Ceftiofur, Tetracycline, Doxycycline, Oxytetracycline, Chlortetracycline, Streptomycin, Dihydrostrptomycinin, Chloramphenicol in raw commingled cow milk above or below tolerance and/or safe levels.



Detection Limits

βLactams

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)


Penicillin G

4

1.5-2

Cefquinome

20

5.0-7


Ampicillin

4

2.0-3

Cefacetrile

125

20-25


Amoxicillin

4

2.0-3

Cefalonium

20

3.0-5


Oxacillin

30

5.0-7

Cefazolin

50

15-20


Cloxacillin

30

3.0-6

Cefadroxil


3.0-5


Dicloxacillin

30

3.0-6

Cefoperazone

60

3.0-5


Nafcillin

30

8.0-10

Cephapirin

‐‐

3.0-5


Benzicillin


3.0-5

Ceftiofur

100

10.0-15


Getacillin


3.0-5

Сefalexin


400-600


Piperacillin


5.0-6

Cefoxazol


50-55


Procaine-penicillin


2.0-3

Cefotaxime


10.0-12



Ticarcillin


4.0-5

Cufuroxime


10.0-12









Tetracyclines

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)


Tetracycline

100

7.0-10

Doxycycline

100

2.0-5


Oxytetracycline

100

710

Chloratetracycline

100

5.0-7


Streptomycin

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)


Streptomycin

200

80-100

Dihydrostreptmycin

200

50-60


Chloramphenicol

MRL (μg/L)

Results (μg/L)

Chloramphenicol

MRL (μg/L)

Results (μg/L)


Chloramphenicol

0.5

0.2-0.3

Chloramphenicol

0.5

0.2-0.3


Chloramphenicol

0.5

0.2-0.3

Chloramphenicol

0.5

0.2-0.3



Milk Sample Preparation

1. Collect milk sample from butch, or farm

2. Make sure the milk has no clot.

3. Milk at room temperature, don’t heat or freeze.

4. Thoroughly mix with the reagent before testing.

 

 

INCUBATOR Preparation

1.     Place the incubator on a flat surface working table.

2.     Connect the charger at the back hole of the machine, then plug at 100-240V.

3.     At the Right side of the incubator, switch the button on, set the desired degree temperature of the machine and wait 15 to stabilized and reach the desired temperature.

4.     When the incubator reach the desired temperature, place the micro-well reagent on the incubator holes and close the lid after.

 

 

 

Test Procedure

1.        Read the manual instruction before testing. Be sure the raw commingled milk and test kit is at room temperature.

2.        Take one Tube from the box, open tube and take out decided number of test to be perform, Use the test kit within an hour beyond time may cause failure of the test. The unused test strip must be sealed and keep out from sunlight and prevent moisture. NOTE: the antigen reagent (sample well reagent) is dried for room temp., don’t think it’s expired or damage.

3.        Aliquots of samples 200ul are taken with the help of pipette doser and transferred into the wells of the test-kit.

4.        Thoroughly dissolve the milk with reagents for 5 minutes till the milk presents pink color. Place wells with the samples into the incubator at 40OC for 5 minutes. It’s allowed to incubate indoors at temperature 16-25 OC for 5 minutes.

5.        Then insert the test strips into the sample-well and incubate them in incubator at 40 OC for 7 minutes. It’s allowed to incubate indoors at temperature 16-25 OC for 7 minutes.

6.        After it remove the test strips from the wells and compare the results of analysis to the table of the results. For storage of the test strip with the result remove the MAX END.