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Beta-lactam+Tetracycline+Sulfonamide’s (TresQ) Rapid Test Kit

Intended Application

The test strip is used for detecting ß‐lactams group, Tetracycline’s & Sulfonamide’s (TresQ) group in raw commingled cow milk above or below tolerance and/or safe levels.


Principle

The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Beta-lactam+Tetracycline+Sulfonamides (TresQ)  in samples, it will combine with marked antibodies, preventing the marked antibodies from combining the (TresQ) antigens of NC nitrocellulose membrane.


【Detection Limits】

β‐Lactams

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)

Penicillin G

4

2‐4

Cefquinome

20

15‐20

Ampicillin

4

3‐4

Cefacetrile

125

100

Amoxicillin

4

4‐5

Cefalonium

20

18‐20

Oxacillin

30

6‐8

Cefazolin

50

40‐50

Cloxacillin

30

6‐8

Cefoperazone

60

50‐60

Dicloxacillin

30

6‐8

Cephapirin

‐-

80

Nafcillin

30

20‐30

Ceftiofur

100

90‐100

Tetracyclines

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)

Tetracycline

100

10

Doxycycline

100

30‐40

Oxytetracycline

100

10

Chlortetracycline

100

30‐40

 

Sulfonamides

MRL (μg/L)

Results (μg/L)

Cephalosporin

MRL (μg/L)

Results (μg/L)

Sulfadoxine

100

35

Sulfabenzamine

100

40

Sulfadiazine

100

35

Sulfamonomethoxine

100

30

Sulfamethoxazole

100

70

Sulfamethoxypyridazine

100

60

Sulfadimethoxine

100

40

Sulfaquinoxaline

100

70

Sulfadimidine

100

25

Sulfachlorpyridazine

100

60

Sulfafurazole

100

100

Sulfamethoxydiazine

100

40


Milk Sample Preparation

1. Collect milk sample from butch, or farm

2. Make sure the milk has no clot.

3. Milk at room temperature, don’t heat or freeze.

4. Thoroughly mix with the reagent before testing.

Test Procedure

1. Read the manual instruction before testing. Be sure the raw commingled milk and test kit is at room temperature.

2. Take one Tube from the box, open tube and take out decided number of test to be perform, Use the test kit within an hour beyond time may cause failure of the test. The unused test strip must be sealed and keep out from sunlight and prevent moisture.

3. Take the milk sample, drop 200ul (about 4-5drops) into the sample-well, and thoroughly dissolve the milk with reagents for 5 minutes till the milk presents pink color.

4. Insert the test strip into the sample-well, read the results after 5 minutes.

Results Judgment

Beta-lactam Tetracycline Sulfonamide’s (TresQ) Rapid Test Kit