25 pieces / bag; 20 bags / box.
Sample preparation
Sample dilutions were prepared as usual (recommended dilutions were sterile saline, D/E neutralized broth, phosphate buffer was not used), and 2-3 serial dilutions were selected based on the number of bacteria in the sample.
2. Vaccination
Place the test piece on the surface of the water platform, uncover the upper film, suck 1mL sample and add the diluted droplets to the culture layer, slowly cover the upper layer film, align the grooved part of the liquid plate to the sample dilution area and place it on the upper layer film. The finger presses the liquid plate to spread the diluent evenly in the pressure ring. 2 repetitions per dilution.
3. Cultivation
The inoculated test pieces were stacked (not more than 15 pieces) in a self-sealing pocket (not sealed), cultured at 36 ° C ± 1 ° C for 24 h ± 2 h; aquatic products cultured at 30 ° C ± 1 ° C for 48 h ± 2 h. The test piece should be placed in a flat place (a small piece of cardboard can be placed in the incubator), and it should not be placed directly on the wire frame to facilitate the deformation of the gel.
4. Count
After the completion of the culture, the test piece was taken out, the red dot on the test piece was counted, and a test piece having a colony number of 20 cfu/piece to 200 cfu/piece was selected for counting. The waste is treated by autoclaving at 121 ° C for 20 minutes.
Fourth, storage
Store in a cool, dark place with a shelf life of 12 months; the opened test piece is stored in a self-sealing pocket at room temperature and used up within 1 month.