Second, the packaging specifications
5 pouch / box.
Third, the test method
Vaccination
The dry powder medium was torn open in a sterile manner, poured into a 500 mL sterile flask, and shaken to uniformly distribute the dry powder medium at the bottom of the flask. Pour 100mL of water into a triangular flask and shake quickly (shake for 3s-5s, do not shake too much to avoid large bubbles in the gel; when the solution becomes viscous, do not shake), let stand for 20min to make the gel completely solidified. If the water sample is highly contaminated, dilute the water sample (take 10mL water sample, add 90mL sterile water to mix), and select the appropriate dilution for testing.
2. Cultivation
The total coliforms were detected in the incubator at 36 °C ± 1 °C for 24 h ± 2 h; the heat-resistant coliforms were detected in a 44.5 ° C ± 0.5 ° C water bath for 24 h ± 2 h.
3. Count
After the completion of the culture, the flask was taken out, and the red dot-folded colony was coliform/heat-resistant coliform, and the unit was CFU/100 mL. If the whole medium turns yellow, there are many red spots and fine bubbles. The waste is treated by autoclaving at 121 ° C for 20 minutes.
Fourth, storage
Store in a cool place away from light and have a shelf life of 1 year.
