【product composition】
Specifications: 10 times / box;
Kit composition:
DATA-------------------------------------------------
Extractant 1 1 bottle Derivatization reagent 1 bottle
Extractant 2 1 bottle Extractant 3 1 bottle
Purifier 1 bottle of furan metabolite complex solution 1 bottle
10 samples of furanone rapid detection card (including micropores)
Negative (-): T-line color development (detection line, near the end of the sample well) is deeper or deeper than the C line (control line), indicating that the concentration of the drug to be tested in the sample is below the detection limit or contains no drug to be tested.The detection process must be carried out in an environment of 20 ° C ~ 30 ° C; try not to touch the
Improved but required equipment
Homogenizer, balance, centrifuge, nitrogen (air) gas blow dryer, vortex mixer, pipette, water bath. [sample processing] Muscle tissue samples (shrimp should be cleaned after removing the head and shell, and the fish should be washed after removing the scales). If it is not detected in time, it should be stored in the dark (within 24 hours). 1. Take a certain amount of de-fat muscle tissue sample and mash it with a homogenizer; 2. Weigh 3g of homogenized sample in a 20ml centrifuge tube, add 200uL of derivatization reagent, then add 4ml of extractant 1 and shake for 2 minutes; Incubate for 30 min at 3.75 °C water bath; 4. After taking out, accurately add 1 ml of extractant 2, then add 5 ml of extractant 3, shake and mix for 3 min, 4000 rpm, and centrifuge for 5 min; 5. Pipette 3 ml of the supernatant into a 5 ml centrifuge tube, and dry at 75 ° C with a nitrogen (air) air blow dryer. 6. Add 1 ml of scavenger to the blown centrifuge tube, oscillate for 30 s, then add 500 uL of nitrofuran metabolite-specific complex solution, mix well for 30 s, 4000 rpm, centrifuge for 5 min (or let stand to Obviously layered); 7. The lower layer solution is the sample liquid to be tested;
