[Detection principle]
The principle of competitive inhibition immunochromatography is applied to the rapid detection of apramycin test strips. Amphomycin in the sample binds to a colloidal gold-labeled specific apramycin monoclonal antibody during the flow process, which inhibits the antibody. The combination with the apramycin-BSA conjugate on the NC membrane detection line, resulting in a change in the color of the detection line. When the sample does not contain apramycin or the concentration is lower than the detection limit, the T line is stronger than the C line. When the concentration of apramycin in the sample is equal to or higher than the detection limit, the color of the T line is significantly weaker than the color of the C line or the color of the T line does not develop or the color of the T line and the C line are not significantly different. Regardless of whether the sample contains apramycin quality control line C line will show color to show that the test is effective.
【product composition】
1 Test strip 8 strips / tube
2 red dry powder micropores 8 holes / tube
3 desiccant 2 pcs / cylinder
4 Instructions 1 copy
[Test steps]
1. Please read the instructions carefully before use. Before use, return the test strip and sample to room temperature.
2. Remove the reagent cartridge, open the required number of microwells and test strips, and mark them. Please use it as soon as possible within 1h. Immediately after the detection reagent is removed, cover the reagent cartridge to prevent it from getting wet.
3. Using a micropipette, pipette 200 μl of the sample to be tested into the microwell, and slowly suck 5 times until the mixture is uniform, and observe no solids with the naked eye (this step is very important).
4. After incubating at 40 ± 2 ℃ for 3 minutes, insert the marked test strip into the microwell (the end of the MAX line printed down, so that it is fully immersed in the solution).
5. Immerse the test strip in the microwell for 6 minutes to interpret the results according to the schematic diagram. The interpretation at other times is invalid.
[Result Judgment]
T-line and C-line color contrast
1.T line> C line, negative, apramycin in the sample is lower than the detection limit
2. T line = C line, weak positive, ampomycin equals the detection limit in the sample
3. T line <C line or T line is not colored, positive, and apramycin is higher than the detection limit in the sample
4. C line is not color, invalid, test strip is invalid, retest
[Result determination diagram]
