[Principle]
This reagent is made by the principle of colloidal gold immunochromatographic test (GICA). After the sample is added to the sample well, it moves along the chromatography membrane with the colloidal gold marker. The object and the antigen on the detection line are bound to show a magenta color. If no CSFV antibody is present in the sample, no color reaction will occur.
[Kit composition]
No. | Name | 50T / box |
1. | test cards (including straws) | 50 |
2. | antibody titer colorimetric card | 1 |
3. | instruction manual | 1 |
[Storage and expiry date] Store in a cool and dry place (2-30 ° C), valid for 24 months.
[Sample requirements]
1. This test card is only suitable for testing pig serum / whole blood samples.
2. Serum: Take 2-3ml of blood according to the conventional method and place it in a clean and dry test tube. Let it stand for about 1 hour. After the blood is coagulated, centrifuge at 4000 rpm for 10 minutes. Serum is naturally precipitated), and the serum is separated. Require serum to be clear, no hemolysis, no pollution. Serum samples can be stored at 2-8 ° C in the short-term and at -20 ° C in the long-term.
3. Whole blood: Whole blood samples without anticoagulant should be tested before coagulation; if partial coagulation has occurred, separate the serum for testing. Anticoagulant whole blood samples should be tested within 24 hours, and do not freeze; freeze-thaw will cause red blood cells to rupture and hemolyze, which will seriously affect the detection effect.
【experiment method】
1. Tear off the aluminum foil packaging bag of the test card, remove the test card, and place it on a flat, clean surface.
2. Use the matched pipette to suck the prepared clarified sample supernatant, and slowly and slowly add 6 drops (about 120ul) into the sample well; 6-7 drops of the whole blood sample.
3. Leave at room temperature for 10-20 minutes to judge the results. Results longer than 30 minutes are invalid.
