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Newcastle disease antibody rapid test card

Introduction

Newcastle disease, also known as Asian chicken mites, is an acute high-level infectious disease of chickens and a variety of birds caused by viruses. The main features are dyspnea, diarrhea, neurological disorders, mucosal and serosal bleeding. Due to the different pathogenic strains, they can be manifested as diseases with large differences in severity.

The Newcastle Disease Virus (NDV) antigen rapid detection card uses a rapid immunochromatographic assay to detect Newcastle disease virus antigens. After the sample to be tested is added to the well, it is moved along the chromatographic membrane along with the colloidal gold-labeled anti-NDV monoclonal antibody. If an NDV antigen is present in the sample, it binds to the antibody on the test line to display wine red. If the NDV antigen is not present in the sample, no color reaction occurs.


Packing specification

20T/box.

Validity period

Storage method : Store at 2 - 30 o C.

Validity: 24 months.

Component

1 quick test card 20 sets

2 sterile cotton swabs 20

3 dropper 20

4 sample buffer 20 bottles

5 disposable sanitary gloves 1 pack

6 instructions 1 copy


Method of operation

1. Use a cotton swab to collect samples from the cloaca (recommended) or the trachea. The cotton swab should be inserted into the cloaca or trachea and repeatedly rubbed on the inner wall. For young birds, fresh faeces can also be taken directly from cotton swabs.

2. Immediately insert the cotton swab into the test tube containing the sample buffer, rotate the cotton swab repeatedly on the test tube wall at least 10 times, and mix the solution to dissolve the sample as much as possible in the solution. Squeeze the cotton swab on the wall of the test tube above the liquid level to allow the liquid to be squeezed out as much as possible and discard the cotton swab.

3. If the particles in the stool are large, allow to stand for 1-2 minutes to precipitate large particles.

4. Remove the test card from the sealed bag. Place the test card on a flat surface, use a pipette to draw the upper clear liquid from the test tube, and slowly and drop 5 drops to the well with the “S” mark.

5. Leave the test at room temperature for 10-15 minutes. If the result is more than 15 minutes, the result is invalid.